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Molecular biologists can no longer afford to be experts in only one par­ ticular area. They need to be equally competent at handling DNA, RNA, and proteins, moving from one area to another as required by the problem. Ad­ vances in the field have been greatly helped by numerous applications of antibody-antigen interactions, so that a basic knowledge of immunology has now become useful. The emphasis of Immunochemical Protocols is not on the production of antibodies, since many people will beg or buy these, but rather on the enor­ mous range of applications to which these powerful reagents can be put. For completeness, however, methods are included for the production of both polyclonal and monoclonal antibodies. Techniques that may be helpful in obtaining antibodies to elusive antigens and to synthetic peptides are also provided. Both qualitative and quantitative methods are described for local­ izing, characterizing, and quantifying various antigens. These range from the use of antibodies in screening DNA libraries to their application in EUSA assays for quantifying adducts in alkylated DNA Each method is described by an author who has regularly used the tech­ in his or her own laboratory. Not all the techniques necessarily repre­ nique sent the state-of-the-art-they are, however, all dependable methods that regularly achieve the desired result. Each chapter starts with a brief description of the basic theory behind the technique being described. The main aim of this book, however, is to outline the practical steps necessary for carrying out the method successfully.

Immunology. Immunopathology --- Molecular biology --- Immunochemistry --- Antibodies --- Biochemistry --- Immunoglobulins --- Immunoproteins --- Chemistry --- Serum Globulins --- Biological Science Disciplines --- Natural Science Disciplines --- Blood Proteins --- Globulins --- Proteins --- Disciplines and Occupations --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Microbiology & Immunology --- Biology --- Health & Biological Sciences --- Methodology --- -577.27 --- Immunity --- Molecular bases of immunity. Molecular immunology --- Methodology. --- 577.27 Molecular bases of immunity. Molecular immunology --- Immunology --- 577.27 --- Medicine. --- Immunology. --- Biomedicine. --- Immunobiology --- Life sciences --- Serology --- Clinical sciences --- Medical profession --- Human biology --- Medical sciences --- Pathology --- Physicians

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Clinical Medicine --- Delivery of Health Care --- Quality of Health Care --- Medical care --- -Delivery of health care --- Delivery of medical care --- Health care --- Health care delivery --- Health services --- Healthcare --- Medical and health care industry --- Medical services --- Personal health services --- Public health --- standards. --- Standards --- -United States. --- -standards. --- Health Care Quality, Access, and Evaluation --- Patient Care Management --- North America --- Medicine --- Health Services Administration --- Health Care --- Americas --- Health Occupations --- Geographic Locations --- Disciplines and Occupations --- Geographicals --- United States --- Physician services utilization. --- Sick --- Use of physicians --- Utilization of physician services --- Visits to physicians --- Delivery of health care --- Visits to physician --- standards --- United States. --- Ambulatory medical care --- Physicians --- Utilization

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Cancer --- Health Services --- Organization and Administration --- Survival Rate --- Health Maintenance Organizations --- Mortality --- Health Services Administration --- United States --- Neoplasms --- Health Occupations --- Health Care Facilities, Manpower, and Services --- Vital Statistics --- Prepaid Health Plans --- North America --- Managed Care Programs --- Health Care --- Group Practice --- Diseases --- Professional Practice --- Insurance, Health --- Disciplines and Occupations --- Data Collection --- Delivery of Health Care --- Americas --- Insurance --- Geographic Locations --- Epidemiologic Methods --- Demography --- Patient Care Management --- Geographicals --- Financing, Organized --- Population Characteristics --- Epidemiologic Measurements --- Investigative Techniques --- Information Science --- Economics --- Public Health --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Environment and Public Health --- Health Care Economics and Organizations --- Oncology --- Medicine --- Health & Biological Sciences --- Prognosis --- Social aspects --- Psychological aspects --- Patients --- mortality --- prevention & control --- therapy

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Littérature psychologique --- Psychologische literatuur --- Databases, Bibliographic --- Reference Books --- bibliography --- Databases, Bibliographic. --- Reference Books. --- 159.9 <01> --- -#GROL:MEDO-159.9:024 --- Psychology literature --- Book, Reference --- Books, Reference --- Reference Book --- Textbooks as Topic --- Bibliographic Database --- Bibliographic Databases --- Database, Bibliographic --- Psychologie: zie ook: Psychiatrie: n-{616.89-008} en n-{615.851}--Bibliografieën. Catalogi --- 159.9 <01> Psychologie--Bibliografieën. Catalogi --- 159.9 <01> Psychologie: zie ook: Psychiatrie: n-{616.89-008} en n-{615.851}--Bibliografieën. Catalogi --- Psychologie--Bibliografieën. Catalogi --- Psychological literature --- Psychology --- Libraries --- Research Design --- #GROL:MEDO-159.9:024 --- Data Adjustment --- Data Reporting --- Design, Experimental --- Designs, Experimental --- Error Sources --- Experimental Designs --- Matched Groups --- Methodology, Research --- Problem Formulation --- Research Methodology --- Research Proposal --- Research Strategy --- Research Technics --- Research Techniques --- Scoring Methods --- Experimental Design --- Adjustment, Data --- Adjustments, Data --- Data Adjustments --- Design, Research --- Designs, Research --- Error Source --- Formulation, Problem --- Formulations, Problem --- Group, Matched --- Groups, Matched --- Matched Group --- Method, Scoring --- Methods, Scoring --- Problem Formulations --- Proposal, Research --- Proposals, Research --- Reporting, Data --- Research Designs --- Research Proposals --- Research Strategies --- Research Technic --- Research Technique --- Scoring Method --- Source, Error --- Sources, Error --- Strategies, Research --- Strategy, Research --- Technic, Research --- Technics, Research --- Technique, Research --- Techniques, Research --- Research --- Clinical Trials Data Monitoring Committees --- Factors, Psychological --- Psychological Factors --- Psychological Side Effects --- Psychologists --- Psychosocial Factors --- Side Effects, Psychological --- Factor, Psychological --- Factor, Psychosocial --- Factors, Psychosocial --- Psychological Factor --- Psychological Side Effect --- Psychologist --- Psychosocial Factor --- Side Effect, Psychological --- Library --- Information Services --- Psychological research --- Behavioral sciences --- Mental philosophy --- Mind --- Science, Mental --- Human biology --- Philosophy --- Soul --- Mental health --- Library resources --- methods --- Research Design. --- Libraries. --- Psychology. --- Psychologie --- bibliography. --- Documentation --- Recherche --- Psychological literature. --- Psychology - Library resources. --- Psychology - Research. --- Science --- Databases as Topic --- Information Centers --- Publication Components --- Investigative Techniques --- Bibliographies as Topic --- Publication Formats --- Books --- Behavioral Sciences --- Information Systems --- Natural Science Disciplines --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Publication Characteristics --- Behavioral Disciplines and Activities --- Publications --- Information Science --- Information Storage and Retrieval --- Handbooks --- Methods --- Bibliography --- Psychiatry and Psychology --- Communications Media --- Disciplines and Occupations --- Medical Informatics Applications --- Medical Informatics --- Social Sciences

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Cross-Cultural Comparison. --- Delivery of Health Care. --- Health Policy. --- Medical Laboratory Science. --- Clinical Laboratory Science --- Medical Laboratory Technology --- Technology, Medical Laboratory --- Clinical Laboratory Sciences --- Laboratory Science, Clinical --- Laboratory Science, Medical --- Laboratory Sciences, Clinical --- Laboratory Sciences, Medical --- Laboratory Technologies, Medical --- Laboratory Technology, Medical --- Medical Laboratory Sciences --- Medical Laboratory Technologies --- Science, Clinical Laboratory --- Science, Medical Laboratory --- Sciences, Clinical Laboratory --- Sciences, Medical Laboratory --- Technologies, Medical Laboratory --- Medical Laboratory Personnel --- Clinical Laboratory Techniques --- National Health Policy --- Health Policies --- Health Policies, National --- Health Policy, National --- National Health Policies --- Policies, Health --- Policies, National Health --- Policy, Health --- Policy, National Health --- Policy Making --- Community-Based Distribution --- Contraceptive Distribution --- Delivery of Healthcare --- Dental Care Delivery --- Distribution, Non-Clinical --- Distribution, Nonclinical --- Distributional Activities --- Healthcare --- Healthcare Delivery --- Healthcare Systems --- Non-Clinical Distribution --- Nonclinical Distribution --- Delivery of Dental Care --- Health Care --- Health Care Delivery --- Health Care Systems --- Activities, Distributional --- Activity, Distributional --- Care, Health --- Community Based Distribution --- Community-Based Distributions --- Contraceptive Distributions --- Deliveries, Healthcare --- Delivery, Dental Care --- Delivery, Health Care --- Delivery, Healthcare --- Distribution, Community-Based --- Distribution, Contraceptive --- Distribution, Non Clinical --- Distributional Activity --- Distributions, Community-Based --- Distributions, Contraceptive --- Distributions, Non-Clinical --- Distributions, Nonclinical --- Health Care System --- Healthcare Deliveries --- Healthcare System --- Non Clinical Distribution --- Non-Clinical Distributions --- Nonclinical Distributions --- System, Health Care --- System, Healthcare --- Systems, Health Care --- Systems, Healthcare --- United States. --- Managed care plans (Medical care) --- Medical care --- Medical technology --- Health Policy --- Delivery of Health Care --- Biomedical Technology --- Cross-Cultural Comparison --- Technology, Medical --- Congresses --- United States --- Culture --- Public Policy --- Allied Health Occupations --- North America --- Patient Care Management --- Health Care Quality, Access, and Evaluation --- Publication Formats --- Technology --- Social Control Policies --- Americas --- Sociology --- Anthropology, Cultural --- Health Occupations --- Health Services Administration --- Technology, Industry, and Agriculture --- Publication Characteristics --- Policy --- Anthropology --- Disciplines and Occupations --- Technology, Industry, Agriculture --- Social Sciences --- Geographic Locations --- Social Control, Formal --- Anthropology, Education, Sociology and Social Phenomena --- Health Care Economics and Organizations --- Geographicals --- Medical Care Plans --- Public Health --- Health & Biological Sciences --- Effect of managed care on --- Health care technology --- Health technology --- Managed care programs (Medical care) --- Managed care systems (Medical care) --- Managed health care --- Plans, Managed care (Medical care) --- Programs, Managed care (Medical care) --- Systems, Managed care (Medical care) --- Medical Laboratory Science --- Health insurance --- Transcultural Studies --- Comparison, Cross-Cultural --- Comparisons, Cross-Cultural --- Cross Cultural Comparison --- Cross-Cultural Comparisons --- Studies, Transcultural --- Study, Transcultural --- Transcultural Study --- Cultural Characteristics --- Healthcare Policy --- Healthcare Policies --- Policy, Healthcare --- Health Care Policies --- Care Policies, Health --- Health Care Policy --- Policies, Health Care --- Policies, Healthcare --- Policy, Health Care